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Image Search Results


Assay IDs of the TaqMan Assays Used

Journal: Investigative Ophthalmology & Visual Science

Article Title: Characteristics of Hyaluronan Metabolism During Myofibroblast Differentiation in Orbital Fibroblasts

doi: 10.1167/iovs.65.13.13

Figure Lengend Snippet: Assay IDs of the TaqMan Assays Used

Article Snippet: Hyaluronidase 1 , HYAL1 , Hs00201046_m1.

Techniques: TaqMan Assay, Migration

mRNA expression of hyaluronidases following treatment with TGF-β for 24 and 72 hours in OFs (non-TED OFs, n = 4; TED OFs, n = 4). ( A ) HYAL1. ( B ) HYAL2. Results are presented as mean ± SEM. **** P < 0.0001.

Journal: Investigative Ophthalmology & Visual Science

Article Title: Characteristics of Hyaluronan Metabolism During Myofibroblast Differentiation in Orbital Fibroblasts

doi: 10.1167/iovs.65.13.13

Figure Lengend Snippet: mRNA expression of hyaluronidases following treatment with TGF-β for 24 and 72 hours in OFs (non-TED OFs, n = 4; TED OFs, n = 4). ( A ) HYAL1. ( B ) HYAL2. Results are presented as mean ± SEM. **** P < 0.0001.

Article Snippet: Hyaluronidase 1 , HYAL1 , Hs00201046_m1.

Techniques: Expressing

Western blot analysis of E-cadherin, ZO-, and Occludin in fibroblasts ( A ) and diabetic skin tissue ( B ). Alterations in the expression of proteins associated with skin tissue’s moisture retention ability, namely, HAS2, HYAL, and AQP3, were examined through western blot analysis in both fibroblast cells ( C ) and skin tissue ( D ). The right bar graphs represent the quantitative results of blots in A – D . Dermal fibroblast cells were cultured in a high-glucose medium for 3 days and treated with umbelliferone at concentrations ranging from 1 to 20 µM. The animal model involved intraperitoneal streptozotocin injections for 5 days, followed by oral administration of umbelliferone for 4 weeks. The indices measured included TEWL (trans-epidermal water loss), a metric quantifying moisture evaporation through the skin ( E ); skin SCH (stratum corneum hydration), a measure of moisture content in the skin’s outermost layer ( F ); and humidity, an indicator of overall skin moisture state ( G ). Measurements were conducted on the dorsal area of the mice ( E – G ). * p < 0.05, ** p < 0.01, *** p < 0.001 for comparisons between the 33 mM glucose/diabetic mice and control groups/control mice; # p < 0.05, ## p < 0.01, ### p < 0.001 for comparisons between the 33 mM glucose/diabetic mice and umbelliferone-treated groups. Statistical significance of the mean values for each group was determined using ANOVA followed by Tukey’s test. Data are expressed as means ± SD

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Umbelliferone alleviates impaired wound healing and skin barrier dysfunction in high glucose-exposed dermal fibroblasts and diabetic skins

doi: 10.1007/s00109-024-02491-z

Figure Lengend Snippet: Western blot analysis of E-cadherin, ZO-, and Occludin in fibroblasts ( A ) and diabetic skin tissue ( B ). Alterations in the expression of proteins associated with skin tissue’s moisture retention ability, namely, HAS2, HYAL, and AQP3, were examined through western blot analysis in both fibroblast cells ( C ) and skin tissue ( D ). The right bar graphs represent the quantitative results of blots in A – D . Dermal fibroblast cells were cultured in a high-glucose medium for 3 days and treated with umbelliferone at concentrations ranging from 1 to 20 µM. The animal model involved intraperitoneal streptozotocin injections for 5 days, followed by oral administration of umbelliferone for 4 weeks. The indices measured included TEWL (trans-epidermal water loss), a metric quantifying moisture evaporation through the skin ( E ); skin SCH (stratum corneum hydration), a measure of moisture content in the skin’s outermost layer ( F ); and humidity, an indicator of overall skin moisture state ( G ). Measurements were conducted on the dorsal area of the mice ( E – G ). * p < 0.05, ** p < 0.01, *** p < 0.001 for comparisons between the 33 mM glucose/diabetic mice and control groups/control mice; # p < 0.05, ## p < 0.01, ### p < 0.001 for comparisons between the 33 mM glucose/diabetic mice and umbelliferone-treated groups. Statistical significance of the mean values for each group was determined using ANOVA followed by Tukey’s test. Data are expressed as means ± SD

Article Snippet: Mouse monoclonal antibodies of collagen I (clone 3G3, catalog no. sc-293182), fibronectin (clone 2775–8, catalog no. sc-69681), pro-collagen I (clone M-60, catalog no. sc-30136), matrix metalloproteinase (MMP)-2 (clone 2C1, catalog no. sc-13594), MMP-9 (clone E-11, catalog no. sc-393859), EVL (clone B-1, catalog no.sc-376943), Fascin-1 (clone 55 K-2, catalog no. sc-21743), VEGF (clone VG-1, catalog no. sc-53462), E-cadherin (clone 5F133, catalog no. sc-71007), Occludin (clone E-5, catalog no. sc-133256), Has-2 (clone C-5, catalog no. sc-365263), HYAL (clone 1D10, catalog no. sc-101340), and AQP-3 (clone F-1, catalog no. sc-518001) were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot, Expressing, Cell Culture, Animal Model, Evaporation, Control